Physioex 91 exercise 3 activity 6 answered research a glucose standard curve is a method for identifying blood glucose levels which was in keeping with my prediction 2 which patient(s) had glucose reading(s) in the diabetic range. The standard diagnosis of diabetes is made when two separate blood tests show that your fasting blood glucose level is greater than or equal to 126 mg/dl the casual plasma glucose test for diabetes the casual plasma glucose test is another method of diagnosing diabetes. A glucose standard curve is a method of monitoring blood glucose over a period to identify patterns and peaks in glucose levels. The trinder method is used to determine glucose concentration only, (lott et al determination of glucose concentration using trinder method biology essay print reference this published: 23rd a standard curve was to be drawn from the absorbencies acquired from the spectrophotometer at. The only way to know how any given insulin works in an individual cat is to perform a glucose curve the standard glucose curve and its alternative a glucose curve is a series of blood glucose determinations made after a dose of insulin is given typically, blood. View lab report - lab 02 - endocrine physiology physioex from mgmt 303 at devry alpharetta week 02 lab endocrine physiology physioex simulation learning outcomes: observe the effects of a pituitary in part i, you will obtain a glucose standard curve. View homework help - physioex chapter 4 exercise 2 from scin 132 at american public university [physioex chapter 4 exercise 2] a the glucose concentration of the sample will be measured and the optical density will be extrapolated from the glucose standard curve.
He endocrine system regulates the functioning of every cell, tissue, and organ in the body betes type ii, glucose standard curve 2 the physioex simulations you will be using in this lab will. Glucose assay there are two glucose assays, using either glucose oxidase (go) or hexokinase (hk) the protocols differ below at the end the hk kit works better for measuring glucose glucose standard curve title: glucose assay author. This site might help you re: what is a glucose standard curve, and how can you use this tool to determine a concentration of glucose. Glucose values measured from the same blood sample using standard reference methods the time of the next insulin injection, the glucose curve should be continued, the scheduled.
Pex-04-02 uploaded by natalie cristine innecken related interests blood sugar insulin diabetes mellitus the optical density of the sample will be measured and the glucose concentration will be extrapolated from the glucose standard curveexperiment results predict question: predict. Exercise4 endocrine system physiology objectives 1 to define the following terms: metabolism, hor- mone replacement therapy, type i diabetes, type ii diabetes, and glucose standard curve 2 to explain the role of thyroxine in maintaining an. Physio ex what is a glucose standard curve metabolic rates were faster than that of the hypophysectomized rat's baseline metabolic rate because it lacked thyroid-stimulating hormones.
Enzymatic method for determining glucose and sucrose (glucose and dilute 20 µl of the 1 nmole/µl standard solution with 180 µl of glucose assay buffer to it is suggested to test several sample dilutions to ensure the readings are within the linear range of the standard curve. A glucose tolerance test measures how well your body is able to process glucose, or sugar your doctor can use it to diagnose diabetes doctors use standard glucose values to diagnose prediabetes, diabetes, and gestational diabetes your doctor will use the following values in milligrams. Glucose assay kit catalog number ka0831 100 assays and fluorescence (ex/em = 535/587 nm) the generated color or fluorescence is directly proportional to the amount apply the corrected absorbance or fluorescence to the glucose standard curve to get b nmol of glucose in the. Answers activity 2 endocrine physioex 91 1 what is a glucose standard curve, and why did you need to obtain one for this experiment did you correctly predict how you would measure the amount of plasma glucose in a patient sample using the glucose standard curve.
Study mathematical sciences 1408 2001 physioex endocrine system notes from veronica p. Lab #3: spectrophotometry background an example of a standard curve generated by experiment: determining the concentration of glucose in today's experiment we will be determining the concentration of monosaccharide glucose in various. After generating a calibration curve using a standard glucose solution and potassium permanganate, you can then determine the concentration of glucose in an unknown solution.
Assessing the shape of the glucose curve during an oral glucose tolerance test otto tschritter, md gold-standard methods, they can be ob- the glucose curve shape of an ogtt was. About glucose curves the glucose curve is a great tool to differentiate between an insufficient insulin dose and the somogyi effect plasma glucose concentrations are measured in the laboratory—the gold standard—or by an in-clinic analyzer. A standard curve, also known as a calibration curve, is a type of graph used as a quantitative research technique multiple samples with known properties are measured and graphed, which then allows the same properties to be determined for unknown samples by interpolation on the graph.
Blood glucose curves in diabetic dogs and cats blood glucose curves are a a useful tool in the stabilisation and monitoring of diabetic animalsthey give an accurate assessment on which to base changes in insulin therapy and are vital in investigating the unstable diabetic. Human anp lab 2 study play what is a glucose standard curve, and why did you need to obtain one for the experimentdid you correctly predict how you would measure the amount of plasma glucose in a patient sample using the glucose standard curve. Background information graph is known as a standard curve you will then use this graph to estimate the glucose concentration in some unknown solutions glucose readily donates electrons which are accepted by the permanganate. Add 20 ul of 400 mg/dl glucose standard to cuvette 6 add 20 ul of draw a best fit line and use this standard curve to determine the glucose concentration for the controls and patient specimens verify that the control results.
Home essays physioex physioex topics: diabetes mellitus what is a glucose standard curve, and why did you need to obtain one for this experiment. Answer to what is a glouese standard curve physioex 90 endocrine system physiology did you correctly predict how you would you measure the amount of plasma glucose in a patient sample using the glucose standard curve. [physioex chapter 4 exercise 2] pex-04-02 ramonistry (25) in physioex the glucose standard curve is a method of monitoring blood glucose over a period to identify patterns and peaks in glucose levels. Glycogen colorimetric/fluorometric assay kit rev 9/13 (catalog # k646-100 100 assays glycogen is a branched glucose polymer, in α-1,4 linkage, with branching via α-1,6 linkage within the standard curve range b. Physioex a c t i v i t y 2 plasma glucose, insulin, and diabetes mellitus 1 - physioex introduction what is a glucose standard curve, and why did you need to obtain one for this experiment.